Internal server error 500 "could not start rstudio in time"

If I am understanding your post and you haven’t done anything & it was working 7 months ago, it probably isn’t anything you did.
RStudio from launched sessions using environmental.yml as the configuration file stopped working, too, see here and the rest of the thread below there.
The root cause hasn’t been found but this adds another piece to the puzzle.

I had posted a workaround for the one with environment.yml to allow accessing the RStudio interface; however, your set-up does not match that and I don’t see a way to apply the trick in your case.

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